Journal: iScience

Article Title: PD-L1 + CD8 + T cells enrichment in lung cancer exerted regulatory function and tumor-promoting tolerance

doi: 10.1016/j.isci.2022.103785

Figure Lengend Snippet:

Article Snippet: anti-human CD107a/LAMP1 -151Eu , Fluidigm , Cat# 3151002B.

Techniques: Recombinant, Antibody Labeling, Enzyme-linked Immunosorbent Assay, Sequencing, Software

Journal: International Journal of Molecular Sciences

Article Title: The Contribution of the Minimal Promoter Element to the Activity of Synthetic Promoters Mediating CAR Expression in the Tumor Microenvironment

doi: 10.3390/ijms23137431

Figure Lengend Snippet: Degranulation against CAR cognate antigen shows a threshold behavior as a function of promoter strength: At 72 h following infection, the human primary T-cells were treated for 48 h with the indicated cytokines (250 U/mL for each cytokine) and for 18 h under hypoxic conditions. Cells were then incubated for 4 h with PBS, anti CD3 OKT3 or ERBB2-FC recombinant protein coated wells. Cells were than washed and stained using CD107a APC or a recombinant ERBB2-Fc. ( A ) Representative FACS plots showing membranal CAR expression by the indicated promoter in the non-stimulated and fully stimulated condition. ( B ) Maximum and background degranulation of human primary T-cells transduced with the indicated promoter controlling the expression of the Herceptin based CAR, showing only the Zs + fraction. ( C ) Average from triplicates of the CD107a + cells from the ZsGreen positive fraction, normalized to the total %ZsGreen positive cells in the indicated treatment and specific promoter.

Article Snippet: Primary human T cells were plated at 5 × 10 4 on 1 μg/mL ERBB2 (R&D) coated 96U plates together with anti-human CD107a APC (Biogems, Rehovot, Israel) and incubated for 4 h in a tissue culture incubator.

Techniques: Infection, Incubation, Recombinant, Staining, Expressing, Transduction

Journal: Current protocols in cytometry

Article Title: CyTOF Measurement of Immunocompetence across Major Immune Cell Types

doi: 10.1002/cpcy.27

Figure Lengend Snippet: Example of a Staining Panel

Article Snippet: 151Eu , CD107a , H4A3 , Fluidigm.

Techniques: Staining